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Microfluidic neuronal cell cultures under hypoxia using the on-chip multi-gas incubation system. Used jacket solutions are without NaHAsc and with 1.0 M NaHAsc. The pCO2 was adjusted to 5% atm based on data from Figure 2(b). Hours denotes the time after seeding. (a and b) Microfluidic cell culture of PC-12 cells. (c and d) On-chip culture of dorsal root ganglion neurons isolated from chick embryo.
