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. 2014 Nov 20;190:76–84. doi: 10.1016/j.jbiotec.2013.12.018

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© 2014 The Authors

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Fig. 5 — Comparison of miRNA transcription in cell lines with endogenous and ectopic up-regulation of Dicer. DUKXB-11 host cells were chosen as reference for the comparison of miRNA expression in response to endogenous (a) and ectopic (b) Dicer up-regulation. (a) Microarray fold changes for 5 selected miRNAs (three positively and two negatively correlated to growth rate) and qPCR fold change of Dicer expression in CHO-K1 and CHO-S cells relative to DUKXB-11 cells. (b) qPCR fold changes for Dicer and the same miRNAs in two pool cell lines exhibiting moderate and strong ectopic overexpression of human Dicer (n = 3, Actb used as reference gene, error bars represent s.d. of mean).