Table 10.

Representative cytochrome P450 (CYP) activities^a in human skin and human reconstructed skin models^b

Activity (preferential for)	Skin	EpiDerm™	Episkin™	Episkin™ FTM	SkinEthik™ RHE	Phenion® FT	StrataTest®
AHH (CYP1 family)	0.24–1.35d
EROD (CYP1 family)	bd-35d 3.0 ± 1.2g	bd/1.7 ± 0.8f	7.8 ± 0.4g	2.6 ± 0.3g	2.8 ± 0.9g	bd	<1d
MROD (CYP1A2)	bd to +	bd/0.7 ± 0.3f
PROD (CYP2B6)	bd to bq	bd/bd				bd	<4d
MFCOD (CYP1/2, especially 2C9)	bd ~≤0.5g	bd/bd	~≤0.5g	~≤0.5g	~≤0.5g
BROD (CYP2B/3A)		bd				bd	<1d
Tolbutamide 4-hydroxylation (CYP2C9)	0.46 ± 0.05e
Bufuralol	bd
1-Hydroxylation (CYP2D6)
Chlorzoxazone 6-hydroxylation (CYP2E1)	2.83 ± 0.34e
Para-nitrophenol hydroxylation (CYP2E1)	bd/+
Midazolam 1-hydroxylation (CYP3A4)	2.35 ± 0.23e
BQOD (CYP3A)	bd-76 ± 41d	94 ± 13d/5.5 ± 0.9f
Erythromycin N-demethylase (CYP3A)	+

More examples and references in the text

AHH aryl hydrocarbon hydroxylase, phenolic benzo[a]pyrene metabolites determined with 3-hydroxy-benzo[a]pyrene as standard, bd below detection, BROD 7-benzyloxyresorufin O-debenzylase, BQOD benzyloxyquinoline O-dealkylase, ECOD 7-ethoxycoumarin O-dealkylase, EROD 7-ethoxyresorufin O-dealkylase, MFCOD 7-methoxy-4-trifluoromethylcoumarin O-dealkylase, MROD 7-methoxyresorufin O-deethylase, PROD 7-pentoxyresorufin O-dealkylase

^aConstitutive; number after slash: induced (highest reported induced activity)

^bFor description of the reconstructed human skin models, see Table 1

^cPrimary keratinocytes in culture (“NHEC,” “normal human epithelial keratinocytes”)

^dpmol/min/mg microsomal protein

^epmol/h/mg microsomal protein

^fpmol/min/mg intact model protein

^gpmol of products/6 h/mg protein (sum of 7,500g supernatant + medium)