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Multiple sizes of uniform hPSC colonies can be formed on µCP SAMs. The patterned substrate spatially defines colony growth within circular features that are either 200 µm (a) or 400 µm (b) diameter. Scale bars = 400 µm. Inset images show patterned colonies are compact with well-defined borders that conform to each micro-patterned SAM spot (Insets, scale bars = 200 µm). c) The uniformity of colony size is consistently maintained on the µCP SAMs. hPSC cell line: H9 hESC (WiCell).
