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. Author manuscript; available in PMC: 2014 Nov 29.
Published in final edited form as: Mater Sci Eng C Mater Biol Appl. 2012 Sep 11;33(1):234–241. doi: 10.1016/j.msec.2012.08.035

Fig. 6.

Fig. 6

(a) FACS analysis indicates that expression of pluripotent stem cell surface markers SSEA-4 (a, upper) and TRA-1–81 (a, lower) for hPSCs (line H9, WiCell) cultured on the chemically defined SAM platform in either conditioned medium (CM) or mTeSR after five passages (P5) is similar to the original P0 population. (b) Additional FACS analysis of the TRA-1–81 positive population indicates that the patterned hPSCs cultured in CM and mTeSR continue to express the pluripotency markers Oct4, Nanog, and Sox2 similar to MEF and Matrigel cultured controls.