Fig. 3.
Levels of DNA adducts formed in pulmonary tissue of rats treated with 5 ppm NNK (●), (S)-NNAL (□) or (R)-NNAL (×) in their drinking water for 10, 30, 50 or 70 weeks. DNA was isolated and hydrolyzed to deoxyribonucleosides or nucleobases which were analyzed by liquid chromatography–tandem mass spectrometry. Panel A, 7-POB-Gua; Panel B, O 2-POB-dThd; Panel C, O 6-POB-dGuo; Panel D, 7-PHB-Gua; Panel E, O 2-PHB-dThd; Panel F, O 6-Me-Gua, Panel G, total POB-DNA adducts; Panel H, total PHB-DNA adducts; Panel I, POB + PHB-DNA adducts.