Fig. 5.

Resveratrol suppressed Kras expression in vivo and in vitro. (A) Western blot analysis of total protein isolated from three APC^CKO/Kras^mut tumors (right panels) collected on day 112 from mice on control diet (Ct), 150 ppm supplemented resveratrol diet (Rv 150), and mice with tumors originally maintained on control diet and switched to 300 ppm resveratrol supplemented diet for 3 weeks (Ct + Rv) with corresponding NAT (left panels) probed with primary antibodies against Kras, pERK, pAkt, pBraf and actin. (B) Western blot analysis of total protein isolated from Kras mutant CRC HCT116 and SW480 cell lines treated with 0 (Ct) or 60 μM of resveratrol in DMSO for 0.5 to 24h probed with primary antibodies against Kras, pERK, pAkt, pBraf and actin. Total protein controls for phospho antibodies were not affected by resveratrol treatment (data not shown). A representative of three repeats is shown (for full length gels, see Supplementary Figures 5–9, available at Carcinogenesis Online).