Figure 9.

Accumulation of Carbohydrates and Intermediates in Energy Metabolism Is Impaired in Closed Limbs of JA-Deficient Flowers.

(A) Relative changes (%) in metabolite accumulation compared with the wild type as detected in closed corolla limbs before anthesis of control (Ctrl) and treated ir-aoc, JMT/mje, and ir-coi1 flowers. Dark colored bars indicate higher levels than the wild type, and light/yellow colored bars indicate lower levels than the wild type. Asterisks indicate significant differences compared with wild-type levels (means ± se; n = 3). Each replicate was obtained from 20+ pooled tissues of three plants. Glucose/Hexoses, combined glucose and hexoses; G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; GADP, glyceraldehyde 3-phosphate; PEP, phosphoenolpyruvate. Derivatized metabolic extracts were analyzed by two-dimensional gas chromatography coupled to mass spectrometry as described (Gaquerel et al., 2009). Asterisks denote significant differences between wild-type and transgenic tissues.

(B) Relative sugar levels (means + se; n = 3) were quantified at the beginning (closed) and after (open) completion of anthesis in control and MeJA- or COR-treated limb tissues. Asterisks indicate significant differences between COR- and MeJA-treated tissues versus control tissues of the same genotype (unpaired t test; *P < 0.05, **P < 0.001, ***P < 0.0001).