Figure 11.

Arabidopsis CDK8 Interacts with Two CycCs.

(A) Strong interactions between CDK8 and two CycCs in split-luciferase complementation assays. Data are mean values ± se from three independent experiments. Error bars indicate se (n = 3).

(B) CDK8 interacts with CycCa and CycCb in Co-IP assays. The experiment was repeated at least three times.

(C) Reduced expression of CycCa and CycCb genes in the cycCab mutant allele. The cycCab allele (SALK_039400C) carries a T-DNA insertion between the CycCa and CycCb genes disrupting the expression of both genes. CycCa and CycCb are tightly linked and transcribed in the same orientation.

(D) A. brassicicola disease symptoms and disease lesion sizes in wild-type, cdk8, and cycCab mutant plants.

(E) B. cinerea disease symptoms and disease lesion sizes in wild-type, cdk8, and cycCab mutant plants.

The disease assays in (C) and (D) were conducted as described in Figure 1. The lesion sizes are mean values ± se from at least 20 disease lesions. Asterisks indicate significant differences (Student’s t test, *P < 0.05, **P < 0.01).