Figure 6.

The cdk8 Mutant Has Increased Cuticle Permeability and a Reduced Cuticular Layer.

(A) Leaves of the cdk8 mutant (right) showing the glossy phenotype.

(B) Enhanced cuticle permeability of cdk8 leaves (right) revealed by toluidine blue staining.

(C) Transmission electron microscopic images of wild-type and cdk8 mutant leaf epidermal cells. The white arrows mark cutin. Bar = 200 nm.

(D) The cdk8 mutant shows enhanced water loss. Water loss was expressed as the percentage of initial fresh weight. Values are averages from 20 leaves for each of three independent experiments. Values shown are means ± se.