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. 2014 Oct 3;26(10):4149–4170. doi: 10.1105/tpc.114.128611

Figure 9.

Figure 9.

The Kinase Activity of CDK8 Is Required for Target Gene Expression.

(A) to (D) PDF1.2 (A), AACT1 (B), CER1 (C), and CER6 (D) gene expression levels in wild-type, cdk8;35S:CDK8-MYC, and cdk8;35S:CDK8D176A-MYC transgenic plants. Lines 7 and 24 are two independent transgenic lines expressing 35S:CDK8-MYC, whereas lines 26 and 28 are 35S:CDK8D176A-MYC lines. Error bars represent se (n = 3).

(E) The kinase-dead CDK8 mutation does not affect the CDK8-MED25 interaction in split-luciferase complementation assays. Error bars represent se (n = 3).

(F) RNAP II recruitment to the PDF1.2 promoter is dependent on the kinase activity of CDK8. Reduced RNAP II recruitment is shown in cdk8;35S:CDK8D176A-MYC transgenic plants compared with cdk8;35S:CDK8-MYC plants after B. cinerea infection. Error bars indicate se (n = 3). ChIP-qPCR experiments were repeated two times with similar results. No Ab, negative control with no antibody.

Statistical analysis was conducted to determine differences in mean values. Asterisks indicate significant differences (Student’s t test, *P < 0.05, **P < 0.01).