Regulation of NRT1.8 Expression in ET/JA Signaling Mutants.
NRT1.8 expression levels in the roots of 4-week-old plants were determined by quantitative RT-PCR, treatments were as indicated, and data were normalized to those of SAND.
(A) and (B)
NRT1.8 expression in roots of ctr1-8
(A), eto1-1
(B), and Col-0.
(C) and (D)
ein2-50
(C), ein3 eil1
(D), and the wild-type control were exposed to 20 μM ACC, 200 μM CdCl2, or 150 mM NaCl for 6 h.
(E)
ora59-1 erf104, tri-1, tri-2, and wild-type plants were treated with 50 μM MeJA or 20 μM ACC + 50 μM MeJA (A+J) for 6 h.
(F)
ein2-50, coi1-1, ein2-50 coi1-1, and wild-type plants were treated with 20 μM ACC, 50 μM MeJA, 20 μM ACC + 50 μM MeJA (A+J), 200 μM CdCl2, or 150 mM NaCl for 6 h.
Values are means ± sd; n = 3 in (A) to (E) and n = 4 in (F).