Figure 3.

Regulation of NRT1.8 Expression in ET/JA Signaling Mutants.

NRT1.8 expression levels in the roots of 4-week-old plants were determined by quantitative RT-PCR, treatments were as indicated, and data were normalized to those of SAND.

(A) and (B) NRT1.8 expression in roots of ctr1-8 (A), eto1-1 (B), and Col-0.

(C) and (D) ein2-50 (C), ein3 eil1 (D), and the wild-type control were exposed to 20 μM ACC, 200 μM CdCl₂, or 150 mM NaCl for 6 h.

(E) ora59-1 erf104, tri-1, tri-2, and wild-type plants were treated with 50 μM MeJA or 20 μM ACC + 50 μM MeJA (A+J) for 6 h.

(F) ein2-50, coi1-1, ein2-50 coi1-1, and wild-type plants were treated with 20 μM ACC, 50 μM MeJA, 20 μM ACC + 50 μM MeJA (A+J), 200 μM CdCl₂, or 150 mM NaCl for 6 h.

Values are means ± sd; n = 3 in (A) to (E) and n = 4 in (F).