T-DNA Insertion Mutant of AP1γ2 Displays Normal Growth.
(A) Diagram of the T-DNA insertion and primers used in RT-PCR.
(B) RT-PCR characterization of ap1γ2. The transcript of AP1γ2 was not detected in the mutant. ACTIN was used as loading control.
(C)
ap1γ2 single mutant showed normal growth compared with wild-type Arabidopsis, ecotype Columbia-0 (Col-0). Bar = 1 cm.
(D) Characterization of hairpin RNAi construct against AP1γ1 in Arabidopsis protoplasts. cFBPase was used as loading control.
[See online article for color version of this figure.]