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. 2014 Oct 28;26(10):4102–4118. doi: 10.1105/tpc.114.129759

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Figure 8. — Dileucine Mediated Tonoplast Trafficking Is Affected When Both AP1 Gamma Adaptins Were Knocked Down.

Tonoplast proteins containing dileucine motif were coexpressed with PM marker RFP-SCAMP1 through particle bombardment. GFP-VIT1 (A) and GFP-INT1 (D) showed tonoplast pattern which separated from RFP-SCAMP1 in wild-type Arabidopsis plants. When RNAi construct against AP1γ1 was coexpressed, GFP-VIT1 ([B] and [C]) and GFP-INT1 ([E] and [F]) were partially targeted to PM showing colocalization with RFP-SCAMP1. The fluorescence intensity profiles along the direction of white arrows were obtained using ImageJ. Bars = 10 μm.