Figure 8.

bHLH090 Specifically Expressed at the Myrosin Lineage Cells in Leaves.

(A) Summary of comparative microarray results showing 32 candidate genes involved in myrosin cell development. See Table 1 for details of these genes. The genes upregulated in syp22-4 are listed in Supplemental Data Set 1. They were compared with the genes upregulated in 4-h estrogen-treated plants expressing ProEstro:FAMA (Hachez et al., 2011).

(B) Quantitative RT-PCR of bHLH090 in 21 d after germination plants of wild-type lines (Col-0) and syp22-4 using Actin2 as a control. Error bars indicate sd (n = 3).

(C) GUS staining of the rosette leaves of ProbHLH090:GUS.

(D) Developmental change in ProbHLH090:GUS expression pattern in the first pair of rosette leaves of the wild-type lines (Col-0) and syp22-4.