Figure 1.

UV-B Enhances HY5 Binding to the Promoters of Its Target Genes MYB12 and CHS.

(A) ChIP of DNA associated with HY5. ChIP-qPCR was performed for the MYB12 and CHS promoters and an intergenic region between the At4g26900 and At4g26910 genes using 10-d-old wild-type plants (Col) or hy5-215 null mutants grown in white light (no UV-B). ChIP was performed with an anti-HY5 antibody or without addition of the antibody (mock). Data shown are representative of five independent experiments.

(B) HY5 ChIP-qPCR using 7-d-old wild-type seedlings grown in weak light and treated with narrowband UV-B for 0.5, 1, and 3 h compared with a −UV-B control.

(C) HY5 ChIP-qPCR using 7-d-old wild-type seedlings grown in weak light and treated for 2 h with different intensities of narrowband UV-B compared with an untreated control (−UV-B).

The numbers of the analyzed DNA fragments indicate the positions of the 5′ base pair of the amplicon relative to the translation start site (referred to as position +1). ChIP efficiency of DNA associated with HY5 is presented as the percentage recovered from the total input DNA (% Input). Error bars represent sd of three technical replicates.