Figure 3.

HY5 Associates with RUP1, RUP2, COP1, and BBX24 but Not with the UVR8 Promoter.

hy5-215, uvr8-6, and wild-type (Col) seedlings were grown for 7 d in a standard growth chamber followed by weak light acclimation for 12 h and narrowband UV-B irradiation for 2 h. ChIP was performed with an anti-HY5 antibody, and copurified DNA was analyzed by qPCR for different primer pairs amplifying parts of the COP1, UVR8, RUP1, RUP2, and BBX24 genomic regions and an intergenic region between genes At4g26900 and At4g26910. The numbers of the analyzed DNA fragments indicate the positions of the 5′ base pair of the amplicon relative to the translation start site (referred to as position +1). ChIP of DNA associated with HY5 is presented as the percentage recovered from the total input DNA (% Input). Data shown are representative of two independent experiments. Error bars represent sd of three technical replicates.