Validation of the Endogenous CAPE1 Identity and the Expression Level Triggered by Wounding and Wounding plus MeJA.
(A) The MS/MS spectra matching the endogenous and synthetic CAPE1 peptide. Red fonts labeled y ions and blue fonts labeled b ions, respectively.
(B) The direct analysis of endogenous CAPE1 expression level in unwounded (UW), mechanically wounded (MW), and wounded plus MeJA (W) treated tomato using LC-SRM-MS represented the means and sd of three biological samples. The statistically significant differences between the unwounded, mechanically wounded, and wounded plus MeJA samples are indicated with three asterisks (P < 0.001) based on Student’s t test.