Table 2.
Differential expression of some significant E. antarcticum B7 genes between the temperatures 0°C and 37°C
| SOLiD (R1-0 vs. R1-37) | Ion torrent (R2,3-0 vs. R2,3-37) | ||
|---|---|---|---|
| Gene ID | Product | log 2FC | log 2FC |
| Cold shock response | |||
| Eab7_1546 | Cold shock protein | 2.61 | 1.94 |
| Eab7_1547 | Cold shock protein | 3.6 | 2.16 |
| Eab7_1548 | Cold shock protein | 2.65 | 2.30 |
| Eab7_1549 | Cold shock protein | 2.59 | 2.46 |
| Eab7_2272 | Cold shock protein | -2.8 | -1.06 |
| Eab7_2747 | Cold-shock DNA-binding domain protein | -1.84 | -1.28 |
| cshA | DEAD-box ATP-dependent RNA helicase CshA | 2.74 | 2.38 |
| cshB | DEAD-box ATP-dependent RNA helicase CshB | 3.37 | 2.92 |
| Transcription, translation and regulation | |||
| nusA | Transcription elongation protein NusA | 2.77 | 3 |
| nusG | Transcription antitermination protein nusG | 2.59 | 2.18 |
| infB | Translation initiation factor IF-2 | 1.95 | 2.37 |
| infC | Translation initiation factor IF-3 | 2.34 | 1.39 |
| rbfA | Ribosome-binding factor A | 1.98 | 2.46 |
| sigW | RNA polymerase sigma factor SigW | -2.26 | -1.6 |
| sigD | RNA polymerase sigma-D factor | 2.6 | 2.14 |
| Protein folding | |||
| Eab7_0447 | Heat shock protein DnaJ domain protein | -3.31 | -1.78 |
| clpB | Chaperone protein ClpB | -3.27 | -1.91 |
| grpE | Protein grpE | -2 | -1.6 |
| dnaK | Chaperone protein DnaK | -2.24 | -1.61 |
| Tig | Trigger factor | 1.49 | 0.93 |
| groL | 60 kDa chaperonin | -3.11 | -2.12 |
| Gros | 10 kDa chaperonin | -3,21 | -2 |
| htpG | Chaperone protein htpG | -5.36 | -3.62 |
| Cell membrane adaptations | |||
| lcfA | Long-chain-fatty-acid—CoA ligase | 1.85 | 1.16 |
| Eab7_0234 | Choline/carnitine/betaine transporter | 3.46 | 7 |
| Energy metabolism | |||
| Eab7_0571 | Cytochrome c oxidase subunit II | -7.54 | -9.6 |
| cbaB | Cytochrome c oxidase subunit I | -6.02 | -9.26 |
| ctaE | Cytochrome c oxidase subunit 3 | -1.72 | -1.99 |
| ctaD | Cytochrome c oxidase subunit 1 | -1 | -0.6 |
| ctaC | Cytochrome c oxidase subunit 2 | -1.68 | -1.32 |
| Pyruvate metabolism – anaerobic | |||
| pflA | Pyruvate formate-lyase activating enzyme | -6.75 | -6.7 |
| pflB | Pyruvate formate-lyase | -7.4 | -7.2 |
| Ldh | L-lactate dehydrogenase | -3.12 | -4 |
| Pyruvate metabolism – aerobic | |||
| pdhD | Dihydrolipoyl dehydrogenase | 1.1 | 1.54 |
| pdhC | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | 1.76 | 2 |