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. 2014 Nov 18;14:291. doi: 10.1186/s12870-014-0291-6

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© Jyothi-Prakash et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Classification of differentially expressed ESTs and expression analysis of selected ESTs. (A) Distribution of ESTs obtained from subtractive hybridization of salt gland rich-tissue and mesophyll tissue from A. officinalis leaves. (B) Expression profile of selected EST’s enriched in salt glands by qRT-PCR analysis of transcripts from mesophyll tissue vs. salt gland rich-tissue. White-brown-complex ABC transporter family (ABC), Ribosomal protein S6 (RP), Dehydrin (DHN), Leucine-rich repeat protein kinase (LR), 3-ketoacyl-CoA synthase (KCS), 1-aminocyclopropane-1-carboxylate oxidase (ACO), Aquaporin (AQP), Transcription factor R2R3 (R2R3), Thioredoxin H (TR), ATP Citrate Lyase (ACL) RQ – Relative quantification (mean ± SE, n = 3).