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. 2014 Sep 13;12:284. doi: 10.1186/1477-7819-12-284

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© Li et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inhibition of cell proliferation and migration of PC-3 cell in response to aquapore 5 (AQP5) silencing. (A) The efficacy of AQP5 siRNA for knockdown of AQP5 mRNA was confirmed by PCR. (B) The efficacy of AQP5 siRNA for knockdown of AQP5 protein was confirmed by Western blotting. (C) Quantification of mRNA. (D) Quantification of protein. (E) Effect of AQP5 silencing on the viability of PC-3 cells. (F) Effects of AQP5 silencing on the invasion of PC-3 cells. Data from at least three independent experiments with duplicate determinations are expressed as means ± SEM. *P < 0.05 was considered statistically significant. (G): Columns in the graph are the diagram of the count analysis.