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. 2014 Nov 24;12:115. doi: 10.1186/1477-7827-12-115

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© Palmerini et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Ultrastructure and organelle distribution in prepubertal (a) and adult (b) ovine cumulus-oocyte-complexes (COCs) after 7 hours of IVM. a) Representative TEM micrograph showing the oocyte with no signs of nuclear reactivation. The nucleus (N) is surrounded by a complete and uniform nuclear envelope (NE) and a round electron-dense nucleolar body (Nu). Bar: 5 μm. b) Representative TEM micrograph showing an ooplasm with several vacuoles (Va) provided with an electron-dense membrane, and a SER close to electron-dense cortical granules (CGs); mv: microvilli. Bar: 1 μm.