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. 2014 Nov 24;12:115. doi: 10.1186/1477-7827-12-115

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© Palmerini et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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General morphology and organelle microtopography in prepubertal (a) and adult (b) ovine cumulus-oocyte-complexes (COCs) after 19 hours of IVM. a) Representative TEM micrograph showing an oocyte cortex with mitochondria (m), provided by numerous cristae. Cortical granules (CGs) are irregularly distributed in the sub-plasmalemmal region; mv: microvilli. Bar: 1 μm. Inset in a): representative LM image showing spikes of the oolemma, associated with CC detachment. Bar: 20 μm. b) Representative TEM micrograph showing an ooplasm with several irregularly shaped vacuoles (Va), mitochondria (m) and multiple layers of electron-dense cortical granules (CGs). Bar: 1 μm. Inset in b): representative LM image showing a regularly round shaped oolemma; mv: microvilli. Bar: 20 μm.