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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Mol Med (Berl). 2014 Aug 15;92(12):1283–1292. doi: 10.1007/s00109-014-1189-3

Fig. 3. Hyperoxia affects HIF-1α downstream targets in angiogenesis pathways.

Fig. 3

(A) Immunoblot analysis of VEGF in the TME. Mice with MCA205 pulmonary tumors were exposed to 21% or 60% oxygen for 72 h; shown are representative samples. (B)(C) Immunohistochemical staining of intratumoral CD31 in mice breathing 21% (left panel) and 60% oxygen (right panel) (20x magnification, P < 0.01, n = 3). Mice with established MCA205 pulmonary tumors were placed in either 21% or 60% oxygen for 72 h. Also shown is the effect of hyperoxic breathing on the levels of the VEGF Receptor (CD309) on tumor cells.