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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Plant Biotechnol J. 2014 Jul 22;12(9):1274–1285. doi: 10.1111/pbi.12224

Figure 3.

Figure 3

Phenotype, tocopherol content and composition in wild-type and transplastomic TMT leaves. (a–b) Transplastomic lines (TMT C.1 and B3.3) showing normal biomass and flowering when compared to wild-type plants. (c) Quantitative data of phenotype from five transplastomic lines and wild-type plants. The plant height and biomass were recorded at the end of their vegetative phase (four-month-old plants) and data presented are means and SD of five individual lines of one representative population. The biomass represents the weight of whole plant excluding the root biomass. (d) tocopherol content and composition in TMT transplastomic and wild-type (WT) tobacco tissues. Tocopherols were extracted in 100% methanol from the mature tobacco leaves and seeds and were quantified using HPLC as described in “Materials and Methods.” The data shown are means and SD from three independent samples.