Table 2.
Brain water content in MCAO model after 3 hr ischemia and 8 hr reperfusion in the right hemisphere under control conditions (only saline injected intraventricularly before the occlusion) and after injection of a NKCC1 inhibitor or β 1- or β 2-adrenergic subtype-specific antagonists, dissolved in saline.
| Left hemisphere | Right hemisphere | |
|---|---|---|
| Saline | 77.97 ± 0.17 (n = 8) | 81.28 ± 0.34 (n = 8)* |
| Ethacrynic | 77.46 ± 0.11 (n = 7) | 78.29 ± 0.76 (n = 7) |
| Betaxolol | 78.02 ± 0.17 (n = 7) | 78.44 ± 0.58 (n = 7) |
| ICI118551 | 77.78 ± 0.32 (n = 7) | 81.08 ± 0.18 (n = 7)* |
In rats with MCAO in the right hemisphere drugs were added 15 min before the occlusion as described in Methods. Water content was calculated as [(wet weight − dry weight)/wet weight] × 100%. In control animals (the same value as in Table 1) an increase in the ipsilateral hemisphere was significant (P < 0.05), as marked with ∗. In the presence of ethacrynic acid or the β 1-adrenergic antagonist betaxolol no significant effect was seen, but in the presence of the β 2-adrenergic antagonist ICI118551 water increased significantly (P < 0.05) in the ipsilateral hemisphere as marked with ∗.