Table 1.
Antibodies for fluorescence immunostaining
| Antibody | Host | Cat. Number or reference | Dilution |
|---|---|---|---|
| Anti-βactin | rabbit | Abcam, ab8227 | 1:1000 |
| Anti-DMC1 | goat | Santa Cruz, sc-8973 | 1:50 |
| Anti-γH2AX | mouse | Upstate, JBW301 | 1:500 |
| Anti-H1T | guinea pig | Handel Lab (Cobb et al. 1999) | 1:1000 |
| Anti-MLH1 | mouse | BD Pharmingen 551091 | 1:50 |
| Anti-PCNA | mouse | Santa Cruz, sc-25280 | 1:500 |
| Anti-SYCP1 | rabbit | Novous Biologicals, NB300-229 | 1:100 |
| Anti-SYCP3 | guinea pig | de Massy Lab | 1:1500 |
| Anti-SYCP3 | rabbit | Chuma Lab | 1:1000 |
| Anti-RNF212 | rabbit | Hunter Lab (Reynolds et al. 2013) | 1:1000 |
| Anti-goat IgG-DyLight 488 | donkey | Jackson Immuno Research, 705-485-003 | 1:500 |
| Anti-guinea pig IgG Alexa Fluor 594 | goat | Invitrogen, A11076 | 1:200 |
| Anti-guinea pig IgG AMCA | goat | Jackson Immuno Research, 106-156-003 | 1:500 |
| Anti-mouse IgG Alexa Fluor 488 | goat | Invitrogen, A21121 | 1:200 |
| Anti-mouse IgG HRP | goat | Santa Cruz, sc-2005 | 1:400 |
| Anti-rabbit IgG Cy3 | donkey | Jackson Immuno Research, 711-165-152 | 1:500 |
| Anti-rabbit IgG HRP | goat | Santa Cruz, sc-2004 | 1:20000 |
| anti-rabbit IgG Alexa Fluor 488 | goat | Invitrogen, A11008 | 1:200 |