The tripartite mAb cocktail was assessed for the ability to protect mice against ricin
(panels A, D-F), SEB (panel B) and ETX (panel C).
All studies involving mice were done in strict compliance the Institutional Animal Care
and Use Committees (IACUC) at the Wadsworth Center, Iowa State University, and University
of California, Davis. (A) BALB/c mice (female, 6-8 weeks of age; Taconic
Labs, Hudson, NY) were housed under conventional, specific pathogen-free conditions.
cPB10, alone or in the cocktail was administered to mice (n=10/group) by intraperitoneal
(i.p.) injection 24 h prior to challenge with 10x LD50 ricin (~2
μg mouse; Vector Laboratories, Burlingame, CA), also by i.p. injection. Survival
was monitored over a period of five days. (B) To evaluate c19F1, the chimeric
mAb alone or in the context of the cocktail was mixed with SEB (1 μg) for 1 hr and
then injected into BALB/c mice (Karauzum et al.,
2012). Four hours later the animals received a potentiating dose of
lipopolysaccharide (40 μg; List Biological Laboratories, Campbell, CA) and were
monitored for survival for 5 days. (C) To evaluate c4D7, the chimeric mAb in
the context of the cocktail was administered to female BALB/c mice by i.p. injection, as
described previously (Garcia et al., 2014).
Twenty-four hours later, the animals were challenged by intravenous injection
3xLD50 ETX.