Figure 3.

Tension-dependent CPC mislocalization in polo mutant meiosis II. In wild-type spermatocytes, the CPC concentrates at the centromeres in metaphase II (a). In polo mutant spermatocytes, we observe defects in CPC localization (b–d). In polo¹/polo¹ metaphase II spermatocytes the CPC is frequently absent from aligned chromosomes (b,c, red arrows) while it is normally concentrated at centromeres of unaligned chromosomes (c, white arrows). (d) In polo¹/polo¹⁰ spermatocytes, the predominant phenotype is a diffuse localization of the CPC all over the chromatin. Green, α-tubulin; red, INCENP; blue, DNA; scale bars, 5 µm.