Figure 4.

Localization of the CPC is abnormal in polo mutant neuroblast mitoses. In wild-type third instar larval neuroblasts, the CPC concentrates at the centromeres in metaphase (a) and transfers to the spindle microtubules and cortex in anaphase (c). In polo⁹/polo¹⁰ mutants, INCENP is dispersed on the chromatin in mitosis (b,d). Green, α-tubulin; red, INCENP; blue, DNA; scale bars, 5 µm. (e) Distribution of cells in the different stages of mitosis and cytokinesis. (f) Frequency of cells showing abnormal CPC localization in different stages of mitosis and cytokinesis (error bars = standard deviation from triplicate experiments).