Fig. 4.

GalT4 regulates glycan complexity in substrate glycoproteins. Substantial increases in tri-antennary and tri-sialylated structures were observed upon GalT4 knockdown. Glycan profiles were measured by HILIC and glycan structures were determined by a combination of exoglycosidase digestions and WAX-HPLC. (A) hCG immuno-purified from CHO cells. (B) hCG immuno-purified from CHO-GnT4-GalT4 cells. Nomenclature of N-glycans is shown in the inset together with a guide to N-glycan monosaccharide symbols, linkage types and positions. For further information on the Oxford notation and nomenclature see http://glycobase.nibrt.ie/glycobase/documents/abbreviations.pdf