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. 2014 Dec 1;127(23):5052–5065. doi: 10.1242/jcs.156059

Fig. 1.

Fig. 1.

Expression of phospho mutants of S36 and S307/S309 in NIH3T3 fibroblasts caused morphological changes. (A) Expression of GFP-fusion WTCAP1 and mutants. Cell lysates from the NIH3T3 stable cells harboring an empty GFP vector, a plasmid expressing GFP-fusion WTCAP1 and the four mutants were resolved on 10% SDS-PAGE followed by western blotting using a GFP antibody. GAPDH was used as a loading control. (B) Effects of phospho mutants on cell size. The area of 50 cells per field were measured individually (using ImageJ software) from three fields for each experiment. Results from three independent experiments were analyzed by using Student's t-test, the error bars represent ±s.e.m; *P<0.05, **P<0.01. (C) Mutations S36A or S307D/S309D reduced the percentage of cells with lamellipodia (LP). Cells with lamellipodia were counted and the percentages were calculated. 100 cells per field were counted from three fields for each cell type, and results from three independent experiments were analyzed and shown as in B.