Table 1.
Summary of the proteins co-purified with Sas3p and Pdp3p identified by mass spectrometry analyses. A portion of eluates of the TAP purifications was analysed by either nano LC–MS/MS Q-TOF (SAS3-TAP) or nano LC MALDI-TOF–TOF (PDP3-TAP). The NuA3 complex components are shown in bold. The proteins associated with Sas3p have been ordered according to the MASCOT protein score; the exponentially modified protein abundance index (emPAI) score is shown in parentheses. (n.d., not determined).
| Protein |
SAS3-TAP |
PDP3-TAP |
||
|---|---|---|---|---|
| Protein sequence coverage (%) | MASCOT protein score (emPAI score) | Protein sequence coverage (%) | MASCOT protein score (emPAI score) | |
| Sas3 | 57.4 | 1633 (1.88) | n.d. | n.d. |
| Nto1 | 54.0 | 981 (1.36) | n.d. | n.d. |
| Pdp3 | 66.8 | 820 (3.56) | 63.8 | 2192 (19.58) |
| Taf30/14 | 87.7 | 555 (2.96) | 69.7 | 156 |
| H2B (HTB1) | 79.4 | 299 (4.58) | 55.7 | 597 (9.64) |
| Yng1 | 57.0 | 256 (1.38) | 15.0 | 26 (0.21) |
| H4 (HHF1) | 78.6 | 215 (2.77) | 49.5 | 157 (1.32) |
| Eaf6 | 56.6 | 208 (3.11) | 28.3 | 34 (0.45) |
| H3 (HHT1) | 73.5 | 144 (0.82) | n.d. | 57 (0.37) |
| H2A (HTA1) | 75.0 | 98 (0.55) | 56.8 | 70 (1.80) |
| Phb2 | 28.1 | 69 (0.20) | n.d. | n.d. |
| Yhz9 | 26.1 | 42 (0.10) | n.d. | n.d. |
| Ubi3 | 11.0 | 31 (0.41) | n.d. | n.d. |
| Rim1 | n.d. | n.d. | 40.0 | 73 (0.87) |
| Taf9 | n.d. | n.d. | n.d. | 28 (0.32) |