Table 2.
Proteins that co-purified with Gcn5p from wild-type or SAS3 deleted cells were identified by a mass spectrometry QSTAR XL analysis. The listed proteins have been ordered according to the MASCOT protein score. The emPAI score offers an approximate, label-free, relative protein quantification in a mixture based on protein coverage by peptide matches in a database search (n.i.: not identified in the Protein Pilot search).
|
GCN5-TAP |
GCN5-TAP sas3Δ |
||||
|---|---|---|---|---|---|
| Protein | Protein sequence coverage (%) | MASCOT protein score (emPAI score) | Protein | Protein sequence coverage (%) | MASCOT protein score (emPAI score) |
| Tra1 | 54.97 | 2399 (0.52) | Ngg1/Ada3 | 83.47 | 1163 (1.65) |
| Ngg1/Ada3 | 83.19 | 1817 (3.31) | Spt7 | 55.25 | 558 (0.24) |
| Spt7 | 74.32 | 1768 (1.65) | Ada2 | 74.20 | 544 (1.00) |
| Taf5 | 67.67 | 1021 (1.38) | Gcn5/Ada4 | 70.39 | 490 (1.25) |
| Gcn5/Ada4 | 82.46 | 988 (3.21) | Taf5 | 62.53 | 483 (0.39) |
| Hfi1/Ada1 | 64.34 | 917 (1,88) | Taf12 | 59.00 | 426 (0.44) |
| Ada2 | 80.65 | 899 (3.56) | Hfi1/Ada1 | 63.73 | 400 (0.60) |
| Spt20 | 64.24 | 841 (1.46) | Taf6 | 64.53 | 313 (0.65) |
| Taf6 | 68.41 | 831 (1.86) | Sgf29 | 78.38 | 275 (0.90) |
| Spt8 | 58.64 | 784 (1.07) | Spt20 | 56.95 | 253 (0.33) |
| Taf12 | 50.83 | 769 (1.31) | Spt8 | 50.66 | 246 (0.34) |
| Sgf73 | 48.55 | 541 (0.49) | Tra1 | 40.84 | 223 (0.05) |
| Sgf29 | 79.92 | 425 (1.12) | Sgf73 | 51.14 | 128 (0.09) |
| Ubp8 | 33.76 | 264 (0.27) | Ahc1 | n.i. | 109 (0.16) |
| Spt3 | 51.93 | 253 (0.64) | Spt3 | n.i. | 98 (0.18) |
| Taf10 | 73.79 | 202 (0.72) | Taf10 | 30.10 | 83 (0.31) |
| Taf9 | 63.06 | 181 (1.04) | Taf9 | 44.58 | 49 (0.20) |
| Ahc1 | 48.59 | 174 (0.22) | |||