Table I.
Genes | Primer sequence | GenBank code | Tm ( o C) |
---|---|---|---|
β1 integrin | FOR: 5′- TGCCTACAACTCTCTTTCTTC-3′ | NM_010578.2 | 58 |
REV: 5′- TGGTTTCAGACTCCTTATTTG-3′ | |||
β-actin | FOR: 5΄- TCCCTGGAGAAGAGCTACG-3΄ | NM_001101 | 66.6 |
REV: 5΄- GTAGTTTCGTGGATGCCACA-3΄ | |||
β3 integrin | FOR: 5΄-TGGAAGAGCCTGAGTGTC-3΄ | NM_016780 | 60 |
REV: 5΄-CGGTAGGTGATATTGGTGAAG-3΄ | |||
αV integrin | FOR: 5΄- GGAACAACGAAGCCTTAG-3΄ | NM_008402 | 55 |
REV: 5΄- GTATCCATCTCTGACTGC-3΄ | |||
α4 integrin | FOR: 5׳- GAATCTCCTCCACCTACTCACAG -3׳ | NM_010576 | 64 |
REV: 5׳-CCAACGGCTACATCAACATATCC-3׳ |