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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Cancer Res. 2011 Nov 11;72(1):165–175. doi: 10.1158/0008-5472.CAN-11-2552

Figure 7. p18INK4c is not required for replicative or oncogene induced senescence.

Figure 7

A. Hs68 cells infected with a retrovirus encoding either shRNA against p18INK4c or a non-specific shRNA (Con) were passaged until they reached M1 senescence. B. Immunoblotting for p18INK4c and p16INK4a in cells infected with p18INK4c shRNA. C. Expression of H-RASG12V in cells transduced with p18INK4c shRNA caused oncogene-induced senescence accompanied by a further reduction of p18INK4c levels and enhanced expression of p16INK4a. D. Lysates from proliferating (P) and senescent (S) fibroblasts were immunoprecipitated with rabbit antibodies against CDK6, CDK4, p18INK4c and p16INK4a. The proteins were fractionated by SDS-PAGE and immunoblotted with mouse monoclonal antibodies against the indicated proteins.

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