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. Author manuscript; available in PMC: 2015 May 27.
Published in final edited form as: Nat Commun. 2014 Nov 27;5:5624. doi: 10.1038/ncomms6624

Figure 2. Induction of TCR-CD8 FRET by agonist on artificial lipid bilayers.

Figure 2

(a) Scheme of artificial lipid bilayers artificial based APC. (b)OT-I hybridomas expressing CD8α wild-type, CD3ζ-eGFP, and CD8β-mCherry (OT-I.ZG.8βR) were added to lipid bilayers containing either H2-Kb-OVA or H2-Kb-VSV monomers and ICAM-1, fixed at the indicated time points and imaged by TIRFM at the immune synapses. Images shown in green represent CD3ζ-eGFP, CD8β-mCherry in red and FRET efficiency in false color scale. Images are representative of three different experiments. Scale bar 5 μm. (c) FRET efficiency analysis of immune synapses from OT-I.ZG.8βR interacting with lipid bilayers containing H2-Kb-OVA (circles). Mean ± s.e.m. of 1 min n=18, 2 min n=33, 3 min n=18, 5 min n=45, 10 min n=50 and 15 min n=52. Or H2-Kb-VSV bilayers (squares), mean ± s.e.m. of 1 min n=12, 2 min n=15, 3 min n=13, 5 min n=21, 10 min n=14 and 15 min n=13. Unpaired t-test. *p<0.01 and **p<0.001.