Figure 2.

An ectopic MEX motif targets an autosomal locus to the nuclear periphery, and the male X chromosome shows widespread interaction with nuclear pores. (A) Location of the MEX motif insertion on chr II, with overlay of LEM-2 ChIP–chip profile (color as in Fig. 1B). (B) Zone 1 scorings for the insertion in A in two strains with a wild-type (WT) and one strain with a mutated rex site in male and hermaphrodite embryos. (C) DamID-seq profile of lamin interaction with the entire C. elegans genome in hermaphrodites and males. Chromosomes are separated by red vertical lines. The signal is averaged over 100-kb windows and normalized by free Dam-GFP control. (D) DamID profile as in C of nucleoporin MEL-28.