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. 2014 Dec 1;28(23):2652–2662. doi: 10.1101/gad.250936.114

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© 2014 Zheng et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — The MSL2 CXC domain specifically recognizes the MRE motif. (A) The CES11D1 MRE motif and derived DNA fragments used in this study. Nucleotides on each strand are numbered, with prime denoting the bottom strand. The dinucleotide sequences recognized by R543 of CXC domains 1–4 in the S15 complex structure are shaded green. Mutations are red. (B) Superimposition of ¹H–¹⁵N HSQC spectra of the ¹⁵N-labeled CXC domain titrated with S12 DNA of the indicated molar ratios. Peaks of R526, G528, and G531 used for fitting are boxed. (C) The combined chemical shift changes Δδ of three residues upon titration of S12 and S12 mutant (S12mut) DNA are globally fit to a binding model with the indicated K_d values.