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Chemical shift perturbation of MRE DNA upon binding of the CXC domain. (A,B) Chemical shift changes of H1′ protons in S12 (A) and CES11D1 (B) DNA induced by binding of one molar equivalent of the CXC domain. Chemical shift changes were calculated as δfree − δbound and are shown in red for the top strand and black for the bottom strand. The CES11D1 sequence is aligned at the bottom, with four R543-binding sites seen in the S15 complex structure shaded.
