Figure 4.

Comparison of TE landscapes indicates dynamics and relatedness of gonadal cell lines. (A) Difference maps of Drosophila chromosome X comparing OSS early and current and OSC early in current cell lines. Each dot represents a TE insertion locus that is either specific to one cell line or commonly shared by both compared lines. Dots for persistent and heterogeneous insertions are offset to aid visualization. Complete D. melanogaster Release 5 reference comparison maps are in Supplemental Figure S7. (B) Tally of total de novo TE insertions (left) and chromosomal breakdown of cell line-specific TE insertions (middle and right). (C) Euler plot showing the greatest overlap in de novo TE insertions between OSC_E and OSC_C cells, greater overlap between OSC cells and OSS_E cells, and unexpectedly low overlap between OSS_C cells and other lines. The overlapping regions are the number of individual TE insertions that are located at the same genomic position between the cell lines. (D) Model for cell line relatedness based on TE landscape similarities and expansion of TEs during prolonged culture of OSS cells.