Fig. 2.

a Concentration-response curve of ghrelin and ANAM in the HEK293/GRLN FLIPR assay. Data are shown as the mean ± SE (n = 3). b Concentration-response curve of ghrelin and ANAM in the binding assay using HEK293/GRLN membrane preparations and [¹²⁵I] ghrelin. Data are shown as the mean ± SE (n = 3). c The effect of ANAM in rat pituitary cell assay in doses ranging from 0.01 nM to 10 μM (n = 4). ANAM anamorelin; FLIPR fluorescent imaging plate reader; GH growth hormone; RLUs relative light units; SE standard error