Table 1.
Stage | Cycle | Step | Temperature | Time |
---|---|---|---|---|
1 | 1 | 1 | 92°C | 3:00 min |
| ||||
2 | 25 | 1 | 92°C | 40 sec |
2 | 50°C | 40 sec | ||
3 | 72°C | 1:00 min | ||
| ||||
3 | 1 | 1 | 92°C | 7:00 min |
2 | 92°C | Hold |
Stage | Cycle | Step | Temperature | Time |
---|---|---|---|---|
1 | 1 | 1 | 92°C | 3:00 min |
| ||||
2 | 25 | 1 | 92°C | 40 sec |
2 | 50°C | 40 sec | ||
3 | 72°C | 1:00 min | ||
| ||||
3 | 1 | 1 | 92°C | 7:00 min |
2 | 92°C | Hold |