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. 2014 Nov 13;2014:462084. doi: 10.1155/2014/462084

Table 1.

Settings of PCR cycle for F. hepatica.

Stage Cycle Step Temperature Time
1 1 1 92°C 3:00 min

2 25 1 92°C 40 sec
2 50°C 40 sec
3 72°C 1:00 min

3 1 1 92°C 7:00 min
2 92°C Hold