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Mineralization assay of osteoblasts. (A) Photomicrographs stained with Alizarin Red S showing increased formation of mineralized nodules of osteoblasts treated with increasing concentrations (10–100 μg/ml) of PG extract at 21 days (B) Quantitative data of Alizarin Red-S extraction expressed in the form of percent calcification. Data represented as mean ± SEM of three independent experiments. **p < 0.01 and ***p < 0.001 as compared with control.
