Table 2.
Primer sequences for identification and detection of the virulence associated genes in P. multocida strains
| Gene | Primer sequence | Accession number | Reference |
|---|---|---|---|
| kmt1 | F - ATCCGCTATTTACCCAGTGG | [GenBank:NC_017027] | [21] |
| R - GCTGTAAACGAACTCGCCAC | |||
| hyaC-hyaD | F - TGCCAAAATCGCAGTCAG | [GenBank:NZ_CM002276] | [22] |
| R - TTGCCATCATTGTCAGTG | |||
| pfhA | F - AGCTGATCAAGTGGTGAAC | [GenBank:NC_016808] | This study |
| R - TGGTACATTGGTGAATGCTG | |||
| nanH | F - GAATATTTGGGCGGCAACA | [GenBank:NC_017764] | [23] |
| R - TTCTCGCCCTGTCATCACT | |||
| hgbA | F - TGGCGGATAGTCATCAAG | [GenBank:NZ_CM002276] | [23] |
| R - CCAAAGAACCACTACCCA | |||
| ompH | F - CGCGTATGAAGGTTTAGGT | [GenBank:NZ_CM001580] | [23] |
| R - TTTAGATTGTGCGTAGTCAAC | |||
| ptfA | F - TCCACTCGTTGTGGCATTCA | [GenBank:NC_017027] | This study |
| R - AGAAACACCTTGAGCTGCGT |