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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Adv Healthc Mater. 2013 Jan 20;2(7):993–1000. doi: 10.1002/adhm.201200334

Figure 2.

Figure 2

(A) Schematic showing experimental setup for characterization of biofilm growth in PLG-coated wells. DMABI was loaded in PLG films solvent-cast on the bottom surface of each well of a microtiter plate and incubated for 24 hours in the presence of a suspension of bacteria. (B) Plot of biofilm growth (as a percentage of growth measured in control wells containing PLG films that did not contain DMABI) as a function of initial DMABI loading. Biofilm growth was characterized and quantified on the bottoms of film-coated wells (closed circles) and at the air/water interface in each well (open circles). (C) Digital pictures of film-coated wells fabricated to contain 18 different loadings ranging from 0.001 to 0.40 µmol DMABI after 24 hours of incubation in the presence of bacteria. Key loading values are labeled; a complete list of all loadings used is included in Table S1. Images shown are of wells after staining of biomass with crystal violet (Row I) and after subsequent de-staining to remove crystal violet (Row II; see text). Results arising from these staining and de-staining procedures were used to quantify amounts of biofilm growth and calculate the results shown in (B). Pictures of wells used as controls containing no polymer coating (bare wells, *) or a polymer coating that did not contain DMABI (PLG only, **) are included for comparison. To aid in interpretation of references to color made in the main text, a color version of this Figure is included as Figure S4 of the Supporting Information.