FIG 1.
(A) Neighbor-joining tree based on percent identity between the published sequences for GRA15 from T. gondii types I, II, and III (GT1, ME49, and VEG, respectively) and that sequenced from H. hammondi HhGer041. (B) Dual-luciferase promoter assay comparing the efficacies of the putative promoters for TgGRA15II and HhGRA15. Data for two trials are shown as the ratios of firefly luciferase signal to T. gondii tubulin promoter-driven Renilla luciferase signal. (C) Western blot showing HA-tagged GRA15 (left) and loading control (ROP5). MW, molecular weight, in thousands. (D) Immunofluorescence assay showing colocalization of HA-tagged HhGRA15 with TgGRA7 in T. gondii strain RH.