FIG 3.

PPG1 is important for filament extension in response to growth under strong filament-inducing conditions. The indicated strains were grown overnight in YEPD medium at 30°C (non-filament-inducing conditions, 0 h time point) and diluted 1:10 into prewarmed YEPD medium plus 10% serum at 37°C (strong filament-inducing conditions). Cells were harvested at the indicated time points (hours), fixed using 4.5% formaldehyde, and washed twice with 1× phosphate-buffered saline (PBS). Images were taken using differential interference contrast (DIC) microscopy. Bar, 10 μm.