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Ppg1 phosphatase activity is important for agar invasion under a variety of filament-inducing conditions. The indicated strains were grown in YEPD at 30°C overnight. A total of 6 × 103 cells of each strain were spotted onto the indicated solid media. Following 2 days of growth at 30°C (or 37°C for the YEPD + serum condition), images were taken before (B) and after (A) each plate was washed with ddH2O.
