FIG 1.
Gyp1 functions in C. albicans hyphal growth. (A) gyp1Δ/Δ and gyp1R292K strains have defects in hyphal development. Yeast cells of BWP17 (WT), HZX201 (gyp1Δ/Δ; for details on strain genotypes, please see Table S2 in the supplemental material), HZX250 (gyp5Δ/Δ), HZX251 (gyp8Δ/Δ), HZX202 (gyp1Δ/Δ:GYP1), and HZX203 (gyp1Δ/Δ:gyp1R292K) were grown overnight in YPD medium at 30°C, adjusted to an OD600 of 0.1, and spotted onto Spider medium plates or Lee's medium plates. The plates were incubated for 3 days or 6 days, respectively. (B) The gyp1Δ/Δ mutant shows markedly slower hyphal extension in liquid media. As shown in the left panel, cells of BWP17 (WT) and HZX201 (gyp1Δ/Δ) were induced for hyphal development in GMM–20% fetal bovine serum (FBS) at 37°C for 2 h before imaging. The right panel shows the distributions of hyphae with different ratios of hyphal length to the diameter of the mother cell body at 2 h of hyphal induction (n = 120).